Regulation of Adenylate Kinase and Creatine Kinase Activities in Myogenic Cells
نویسندگان
چکیده
منابع مشابه
Regulation of adenylate kinase and creatine kinase activities in myogenic cells.
The regulation of the specific activities of adenylate kinase (EC 2.7.4.3) and creatine kinase (EC 2.7.3.2) in myogenic cell lines is independent of cell fusion. The observed increases in enzyme specific activities are cell density dependent, and may be further broken down into contributions from an increase in enzyme activity per cell and a decrease in protein per cell. Only the former appears...
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The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
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Elemental sulfur is a specific and potent inhibitor of the muscle-type isoenzyme of adenylate kinase (EC 2.7.4.3). We find inhibition by sulfur and by diadenosine pentaphosphate to be similarly potent and specific. Some properties of inhibition of adenylate kinase isoenzymes by sulfur are given. The adenylate kinase isoenzymes from skeletal muscle, brain, and heart muscle are inhibited by sulfu...
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At various intervals after plating, cultures were exposed to 2-h pulses of tritium-labeled leucine. For determination of incorporated radioactivity, the creatine kinases were isolated by specific immunoprecipitation. Sodium dodecyl sulfate-gel electrophoresis was used to separate the two types of creatine kinase subunits MCK and B-CK from each other and their radioactivity was determined in the...
متن کاملThe effect of waterpipe smoking and chronic immobilization stress on serum creatine kinase and alkaline phosphatase activities in female rats
Background: Various studies indicate that there is association between life style and various enzyme activities. This study was conducted to determine the effects of waterpipe smoking and immobilization on serum creatine kinase and alkaline phosphatase activities in female rats. Methods: In this experimental study female Wistar rats were divided into control, waterpipe smoking, immobilized...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.6.2377